Urine Metabolomics

Urine metabolomics has recently emerged as a prominent field for the discovery of non-invasive biomarkers that can detect subtle metabolic discrepancies in response to a specific disease or therapeutic intervention. Urine, compared to other biofluids, is characterized by its ease of collection, richness in metabolites and its ability to reflect imbalances of all biochemical pathways within the body. Urine collection for metabolomic analysis, samples must be immediately frozen to quench any biogenic and/or non-biogenic chemical reactions. Combined with the qualitative and quantitative capabilities of MS, and due to the continuous improvements in its related technologies liquid chromatography (LC)-MS is unequivocally the most utilized and the most informative analytical tool employed in urine metabolomics. The Urine Metabolome database is a freely available electronic database containing detailed information about ~3100 small molecule metabolites found in human urine along with ~3900 concentration values. Each metabolite entry contains more than 110 data fields and many of them are hyperlinked to other databases (KEGG, PubChem, ChEBI, Chemspider, DrugBank, PDB and Uniprot). The information includes literature and experimentally derived chemical data, clinical data and molecular/biochemistry data.

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