Yokohama City University Graduate School of Medicine, Japan
Title: Molecular Mechanism for modulationof a multiple transcriptionfactorcomplexformed on enhancer site upon phosphorylation
Biography: Kazuhiro Ogata
Transcriptional regulationis a fundamental mechanism for cell proliferation and differentiation. A major player in this process is a member of transcription factors (TFs).Activities of TFs are modulated by chemical modifications such as phosphorylation through cell signaling pathways directing cell functions. Molecular mechanisms of regulation for the functional high-order assemblyconsisting of multiple TFs and enhancer DNA (enhanceosome) under cellsignaling are largely unknown. So far, we investigated changes in assembly state of an enhance osome upon a TF’s phosphorylation, using crystallographical, biophysical, and molecular dynamic alanalyses. Here, wewill illustrate the case of Ets1 in non-phosphorylated and phosphorylated forms on its target gene enhancers with or withoutits partner TFs, Runx1/CBF or Pax5, as an example. A structure-function relationship of a TF-DNA assembly and an effect by phosphorylationwill be discussed.