Khan Farheen Badrealam
National Dairy Research Institute, India
Title: iTRAQ-based analysis of complex serum proteome alterations in Indian zebu cattle subjected to heat stress
Biography
Biography: Khan Farheen Badrealam
Abstract
Heat stress is a major stressor, which affects animal’s performance substantially and molecular basis coherent with the ability of Bos Indicus to survive and adapt to heat stress. Reckoning with these, the present study was planned to examine the complex serum proteome alterations in Indian zebu cattle following heat stress and normothermia applying isobaric Tags for Relative and Absolute Quantification (iTRAQ) proteomics approaches. The data of the present study suggests that out of the total 144 proteins identified, 53 proteins were differentially expressed after 4 h of heat stress (23 proteins upregulated and 30 proteins downregulated), whereas 48 (23 proteins upregulated whereas 25 downregulated) and 51 (18 proteins upregulated whereas 33 downregulated) proteins were differentially expressed after 24 and 48h of recovery period respectively. Intriguingly, this is the seminal report investigating the differential proteome profile of Sahiwal serum samples in response to heat stress. The identified proteins were subjected to analysis for their prospective involvement in various biological, cellular and molecular processes including thermoregulatory mechanism thereof. The high confidence dataset generated herein may plausibly foster identification of prospective biomarkers related to heat stress in Indian zebu cattle; nonetheless, the findings of the present study significantly advances our understanding of the physiological aspects of heat stress induced responses in Sahiwal cattle